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antislc3a2 #13180  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc antislc3a2 #13180
    Antislc3a2 #13180, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antislc3a2 #13180/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    antislc3a2 #13180 - by Bioz Stars, 2026-03
    90/100 stars

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    Effects of porcine pericardial decellularized matrix bilayer patches containing exosomes (dECM + collagen + Exo) on cells in vitro. (A) Morphological observations of three cell types after 1, 3, and 7 days of growth on the surface of bilayer matrix patches, (green: cytoskeleton, blue: DAPI). (B) Cells' viability after co-incubation of dECM + collagen + Exo and human skin fibroblasts, keratinocytes and HUVEC. (C) Transwell images of human skin fibroblasts when co-cultured with dECM + collagen + Exo bilayer patches. (D) dECM + collagen + Exo bilayer patches induced HUVEC formation tubes. (E) Statistical analysis with tube-forming branches (n = 5). (F) Western Blott evaluation of proliferation-related proteins (Ki67) and angiopoiesis-related proteins (VEGF) expressed by fibroblasts and HUVEC, respectively, n = 3, statistical significance was obtained by one-way ANOVA, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Materials Today Bio

    Article Title: Porcine pericardial decellularized matrix bilayer patch containing adipose stem cell-derived exosomes for the treatment of diabetic wounds

    doi: 10.1016/j.mtbio.2024.101398

    Figure Lengend Snippet: Effects of porcine pericardial decellularized matrix bilayer patches containing exosomes (dECM + collagen + Exo) on cells in vitro. (A) Morphological observations of three cell types after 1, 3, and 7 days of growth on the surface of bilayer matrix patches, (green: cytoskeleton, blue: DAPI). (B) Cells' viability after co-incubation of dECM + collagen + Exo and human skin fibroblasts, keratinocytes and HUVEC. (C) Transwell images of human skin fibroblasts when co-cultured with dECM + collagen + Exo bilayer patches. (D) dECM + collagen + Exo bilayer patches induced HUVEC formation tubes. (E) Statistical analysis with tube-forming branches (n = 5). (F) Western Blott evaluation of proliferation-related proteins (Ki67) and angiopoiesis-related proteins (VEGF) expressed by fibroblasts and HUVEC, respectively, n = 3, statistical significance was obtained by one-way ANOVA, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: The primary antibodies used were as follows: Ki67 (13180-T48, Sino Biological Inc, China), 1:500; VEGF (ab32152, Abcam, USA), 1:2000; Atp1a2 (ab166888, Abcam, USA), 1:2000; Calm4 (CSB-PA004454GA01HU, Cusabio), 1:1000; Gngb1 (PA5-101543, Invitrogen), 1:1000; GAPDH (#2118S, CST), 1:1000.

    Techniques: In Vitro, Incubation, Cell Culture, Western Blot